Biotechnological methods of plant improvement 04-TZR-TPR-BMPI-SD2
Lecture:
Base of traits inheritance. Gene expression – from a gene to protein. In vitro cultures – micropropagation, somatic hybridization, production of polyploids. Flow cytometric analysis of ploidy and genome size stability. Direct and indirect methods of plant transformation. Genetically modified (GM) food – pros and cons. Legal regulations on GM food.
Laboratories:
In vitro plant tissue culture. Micropropagation of medical and crop plants. DNA extraction from fresh and processed plant food products. Electrophoresis. Spectrophotometric analysis of DNA quality and quantity. PCR and its application in GMO detection in food products. Flow cytometry.
Koordynatorzy przedmiotu
W cyklu 2017/18Z: | W cyklu 2019/20Z: | W cyklu 2020/21Z: | W cyklu 2018/19Z: | W cyklu 2021/22Z: | W cyklu 2019/20L: | W cyklu 2016/17Z: |
Efekty kształcenia
On successful completion of the course student is supposed to know principles of genetics, to characterize basic techniques applied in plant biotechnology, such as micropropagation, DNA extraction from fresh and proceeded plant food products, electrophoresis, DNA spectrophotometry, polymerase chain reaction (PCR), flow cytometry. Student possesses knowledge on plant in vitro cultures, methods of plant genetic transformation and techniques of genetically modified organisms (GMO) detection in plant food products. He/she knows legal regulations on GM food. On successful completion of the course student is supposed to be able to work in biotechnological laboratory and use biotechnological techniques. Student will learn how to find, understand and use professional literature on plant biotechnology studies. Student will be able to communicate in English in foreign biotechnological laboratories. On successful completion of the course student will be able to cooperate in international teams working in the field of plant biotechnology.
Literatura
Basic literature:
1. Klug W.S., Cummings M.R., Spencer Ch.A., Palladino M.A. 2012. Concepts of Genetics. Pearson Education Inc.
2. Steward C.N., Touraev A., Citovsky V., Tzifira T. 2011. Plant Transformation Technologies. John Wiley & Sons
3. Internet sources
Supplementary literature:
1. Scientific papers.
Więcej informacji
Dodatkowe informacje (np. o kalendarzu rejestracji, prowadzących zajęcia, lokalizacji i terminach zajęć) mogą być dostępne w serwisie USOSweb: